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Xu Y, Lv D, Yan C, Su H, Zhang X, Shi Y, et al

Lysates were then centrifuged for 15 min at 175,000 g at 4 C

Immobilized metal ion affinity chromatography on Co 2 + carboxymethylaspartateagarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle

The clinical frailty index was assessed as described previously [27]