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A 12-week randomized trial demonstrated: Average weight loss of 2.6 kg in AOD-9604 group (1 mg/day) versus 0.8 kg in placebo group Preferential reduction in abdominal and visceral fat deposits No adverse effects on carbohydrate metabolism or insulin sensitivity Non-dose-dependent effects, with higher doses (10 mg/day) producing less weight loss than lower doses However, a subsequent 24-week Phase III trial with 502 subjects incorporating intensive diet and exercise showed no statistically significant weight loss compared to placebo, leading to development termination in 2007

They compared a target of 4.0 g/day against typical European intakes which range from 4.2 to 8.6 g/day in adults and believed 4.0 g/day to be a practical amount

Beta-3-AR knockout mice were completely unresponsive to chronic lipolytic effects, demonstrating the essential role of this receptor pathway