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Inflammasome and Mitochondrial Status Assessment by Imaging Flow Cytometry Inflammasome complex assembly was evaluated by detection of ASC speck formation by imaging flow cytometry, as previously described ( Flow Cytometry PBMCs were stained with the following list of fluorescently labeled antibodies against cell surface markers subsequently detected by flow cytometry: anti-CD14 BV605 (clone: M5E2), anti-CD16 PE-Cy7/BV711 (clone: 3G8), and anti-CD3 PE (clone: HIT3a) from BioLegend