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Subsequently, the RAW264.7 cells were washed with PBS and stained by Ca 2+ Probe Fluo-4 AM for 20 min

The key difference lies in their molecular structure: GHK-Cu contains glycine, histidine, and lysine, whereas AHK-Cu replaces glycine with alanine

The latter is encoded by UGA and directed by the selenocysteine insertion sequence, SECIS element [28, 29], which serves as a platform for the recruitment of elongation factors of selenocysteine-tRNA [Ser]Sec (Sec-tRNA [Ser] Sec ) translation that decodes the UGA codon for the whole family of selenoproteins