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Description

Int J Clin Pharmacol Ther 37 , 529547 (1999)

Physical characteristics and aerosol performance of naringin dry powders for pulmonary delivery prepared by spray-drying

All steps were the same as for the 96-well plate assay except that the culture volume was 1 mL, the volumes of the fixative, staining solution, and acetic acid were scaled proportionally (0.5 mL fixative, 1.25 mL of crystal violet solution, and 1.25 mL of glacial acetic acid per tube), and the washes were performed by gentle pipetting (2 mL water per tube per wash) instead of by submersion