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336 Several underlying factors, such as differences in the experimental design, model systems, or patient heterogeneity, may account for the differences in the mechanisms of action of CP31398 and APR-246 and cause different therapeutic responses

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Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2