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To ensure a well-defined Caco-2 cell monolayer prior to initiation of experiments, aliquots of the cell buffers were analyzed by fluorescence to determine the transport of the impermeable dye Lucifer yellow

Both this subgroup and the 18 subjects with a normal Schilling test had low serum cobalamin concentrations at baseline, which increased significantly to normal range within 4 weeks of treatment [276 69 vs 96 15 pmol l 1 (CI 95% 130.5, 231.3)

As a result, these studies help scientists better understand how neurotransmitter systems interact within complex biological models

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