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Glutathione standards of 0, 2.5, 5, 10 M and 100 M of both GSH and GSSG were prepared and combined in the following ratios to a final volume of 90 L: 10 M GSH: 0 M GSSG, 9 M GSH: 1 M GSSG, 8 M GSH: 2 M GSSG, 6 M GSH: 4 M GSSG, 4 M GSH: 6 M GSSG, 2 M GSH: 8 M GSSG, 1 M GSH: 9 M GSSG, and 0 M GSH: 10 M GSSG