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T cell populations in CKD exhibit a shift toward pro-inflammatory phenotypes, with reduced regulatory T cell function, which fails to suppress excessive immune responses, thereby sustaining inflammation and tissue injury ( FIGURE 2 Dysbiosis of the gut microbiota leads to the disruption of tryptophan metabolism (resulting in the production of toxins such as IS and the entry of lipopolysaccharides (LPS) into the bloodstream (40)

however, because of the higher presence of intracellular proteins (cytoskeletal proteins in the T cell and CAR-T cell cocultures and mitochondrial proteins in the TCR-T cell cocultures), we applied a less stringent cutoff (10% instead of 5% FDR) (Supplementary Fig

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In addition, eliminating a buffer allows the method to be easily adapted for other complex samples analysis of NAC in the future