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For indirect calorimetric analyses, heat production, locomotor activity, O 2 consumption (VO 2 ), CO 2 production (VCO 2 ), and RER (defined as VCO 2 /VO 2 ) were monitored over 48 h after adaptation of the mice to individual metabolic cages and were assessed by using the Comprehensive Laboratory Animal Monitoring system (Columbus Instrument, Columbus, OH)