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Some studies noted mild dehydration in treated animals

Longevity protocol: Dose: 0.5-1mg daily (lower end) Frequency: 3-5 times weekly Cycle: 8 weeks on, 4 weeks off, indefinite repetition Combine with: Overall health optimization (sleep, nutrition, exercise) Lower doses for longevity reduce cost and theoretical concerns about long-term copper peptide use while potentially maintaining meaningful benefits

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This induction was less than the Gcn4 protein seen when cells were nitrogen starved, consistent with the fact that autophagy flux induced by V-ATPase-dependent autophagy was lower than that observed during nitrogen starvation