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In addition, since L-theanine was shown to have a relaxing effect and gamma-aminobutyric acid (GABA) was an important inhibitory neurotransmitter, the mixture of L-theanine and GABA had a positive synergistic effect on sleep behavior, including sleep quality and duration in caffeine-induced awake rats, and the mechanism might be that the mixture could promote the expression of GABA receptor, which was conducive to sleep (38)

Immediately after, read and record absorbance at 340nm every minute for 5 time points at 25C

After about three months, when new roots have grown, the branch can be cut from the mother plant

PMID: 56 LaitmanB