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Additionally, the authors found that an overexpression of the GhrR led to a weak or strong anxiolytic effect, depending on the expression level in the lateral nuclei of the amygdala, which could indicate that GhrR effects on anxiety depend on the nuclei in which it is expressed ( 6.4 Gut Motility AN patients suffer from an impairment in gut motility, which is reported as one of the main factors reducing the quality of life (107, 108)

Cells were lysed in 50 mM Tris pH 7.5, 200 mM NaCl, 5 mM DTT, 4 mM MgCl2 and the soluble lysate fraction incubated with Glutathione Sepharose 4B resin (Cytiva) for 2 hours at 4 C

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