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[DOI] [PMC free article] [PubMed] [Google Scholar] 141.Arcos M., Goodla L., Kim H., Desai S.P., Liu R., Yin K., Liu Z., Martin D.R., Xue X

Dement Geriatr Cogn Disord 25(6):531538

This allows greatest stabilization of developing negative charge at C as bond cleavage occurs, and additionally allows hyperconjugation of the aligned bond in the ground state

Blocking solution was removed and first antibody diluted in 0.5% BSA in PBS was added to the samples (1:30001:10000 for HNEJ2, 1:500 anti-activated caspase-3, Calbiochem)