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The supernatant was replaced by 35 mL of isotonic resealing medium (NaCl 145 mM, HEPES 5 mM, glucose 5 mM, pH 7.4, 300 mOsm/kg H2O , 37C)

Minchin et al

Early forms have almost disappeared from use as a consequence of their side effects, which include the 'cheese reaction' that is, stimulation of cardiovascular sympathetic nervous system activity due to a build-up of dietary amines