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doi: 10.1038/s41467-022-31390-5 39 VatanenTFranzosaEASchwagerRTripathiSArthurTDVehikKet al

vial swirled (not shaken) for 3060 seconds, since shaking introduces foam that can denature the peptide

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Data were searched with both Sequest HT and MS Amanda 3.0 against a UniProt mouse complete proteome database (2022) and corresponding decoy reversed protein sequences