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Mass spectra were acquired using a Data Dependent Acquisition mode (DDA) with full scan spectra done in the Orbitrap while fragments ions were generated by Higher energy Collision-induced Dissociation (HCD) and measured in the linear ion trap

Draw the appropriate amount of bacteriostatic water into your syringe

Storage: Refrigerate after mixing and use within ~4 weeks (common handling guideline)

ACI, aortic calcification index Taken together, there are conflicting results from various sources, such as the uncertainty of sKlotho assays or Klotho production/clearance and differences in disease conditions, treatment modalities, drugs, and study quality