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Each extraction buffer contained the following protease inhibitors: 1 mM 1,10-phenanthroline, 1 mM phenylmethanesulfonyl fluoride, 334 M 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, 14 M E-64, 5.25 M leupeptin, 3 M bestatin, and 1 M pepstatin A

The inconsistent results may be related with several reasons: mRNA stability, transcription factor activity, alternatively splicing, ribosome loading and translation efficiency, post-translational regulation, cell-cycle dependence, and environmental factors, etc

The inclusion criteria for TNBC were based on the following immunohistochemical results: i) ER, PR and HER2 were negative

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