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In brief, the pyocyanin-ascorbic acid mixture was mixed with chloroform (Univar., Ingleburn, Australia) to a dilution factor of 300 L of chloroform for every 1,000 L of the mixture in 15 mL falcon tubes and vigorously shaken manually and vortexed immediately for 5 s followed by centrifugation at 4500 g for 10 min at 4C

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Interestingly, several Rab GTPases were only observed in the oleic acid condition

Independent samples t -tests were used for comparisons between two groups and one-way ANOVA followed by Bonferronis post hoc test was employed for comparisons among multiple groups