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However, our team recommends refrigerating them upon arrival for protocol consistency and to prevent accidental exposure to heat

Future research should employ validated measurement tools to ensure reliable results and facilitate comparisons across different studies

Changhan Lee et al, IGF-I regulates the age-dependent signaling peptide humanin

A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)