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Under certain conditions, THP-1 cells were co-cultured with GMSCs for 24 h followed by stimulation with 100 ng/mL of LPS for 24 h to induce M1 macrophages

When the cell culture reached an OD 600nm of 0.7, PttGSTF1 expression was induced by the addition of 0.1 mM isopropyl -D-1-thiogalactopyranoside (IPTG) and cells were further grown for 4 h

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10.1186/s12967-025-06300-0 Summary Keywords exercise, ferroptosis, neurodegenerative disease, exerkine, iron metabolism Citation Tang S, Zhang J, Chen J, Zhou Z and Lin Q (2025) Ferroptosis in neurodegenerative diseases: potential mechanisms of exercise intervention