glutathione does what Is the Master Antioxidant? Here’s Science Says Glutathione: The Master Antioxidant Your

As an effect of ROS on the central nervous system (CNS), which has a high lipid content and high oxygen consumption, the oxidative agents promote lipid peroxidation, leading to membrane fluid loss, suppressing membrane potential, and increasing the accumulation of ions, particularly Ca 2+

After sustained tone was established, BPC 157 was added cumulatively to the bath

Proc Natl Acad Sci 99:16041609 Rothstein JD, Van Kammen M, Levey AI et al (1995) Selective loss of glial glutamate transporter GLT-1 in amyotrophic lateral sclerosis

Following another 48-h incubation, the media was removed and 250 l of RIPA buffer (50 mM Tris, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP-40, 0.1% SDS, pH 8) was added to each well