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These findings suggest that in ES cells, Arg and Leu deprivation converge on a common adaptive program

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5eh) were analyzed to assess their expression levels, indicated by brown staining in the nuclei or cytoplasm of spermatogenic cells

To ask how -KG insufficiency depletes the cytosolic NADPH pool, we first confirmed that cytosolic NADPH-producing enzymes ME1 and IDH1 are essential for cell survival during energy stress, as previously reported 26,31 , because knockdown of either gene sensitized Huh7 and H1299 cells to glucose starvation (Supplementary Fig