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The dried samples were reconstituted in a solution of IPA/ACN/water (4:3:1

hydrocortisone lotion in the treatment of childhood atopic dermatitis: a pilot study

After incubation with primary antibodies followed by horseradish peroxidase-linked secondary antibody, the target protein was visualized by Immobilon Western Chemiluminescent HRP substrates (Millipore)