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The intrinsic pathway is controlled by the BCL2 protein family, which regulates apoptosis sensitivity through the control of mitochondrial membrane permeability and subsequent release of mitochondrial proteins, such as CYCS (cytochrome C, somatic) and DIABLO/SMAC (diablo IAP-binding mitochondrial protein) [99]

The PCR protocol consisted of: a pre-heating step at 95C for 1.5 min, 35 cycles of heating at 95C for 30 seconds, cooling at 64C for 30 seconds, elongation at 72C at a rate of 1 kb/min, and a final elongation step of 5 minutes at 72C