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We can get a sense of this if we look at the death-response curve: As Campbell and Dunaif noted, The total dose for the highest dose group (4.0 mg/kg) approaches the median lethal dose of 5.0 mg/kg for male F344 rats. The median lethal dose, or LD 50 , is the dose required to kill half the animals

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CpG-ODNs activate plasmacytoid dendritic cells and B cells via the TLR9MyD88NF-B pathway, inducing CD80/CD86 expression and cytokine secretion (e.g., type I interferons, IL-12), which enhance T-cell priming and systemic antitumor immunity

Blood-brain interfaces: relevance to cerebral drug metabolism