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Rapid determination and novel optimisation method for berberine extraction has been developed by Soxhlet extraction utilising central composite design-response surface methodology (CCD-RSM)

doi: 10.1016/j.lfs.2015.01.006, 118

With respect to artifacts, a comparison of the ligands bound when At GSTF2 was recovered from being expressed transiently in Nicotiana with those retained by the enzyme when solvent extracts from the identical plant tissue were passed over the immobilized protein, strongly suggested that the glutathionylated lignanamides ( 5, 6, 7 ) were artifacts formed during extraction

After incubation, absorption at 450 nm was measured and translated to the cell viability with a standard curve