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Using glycine as an example, with a carboxyl group p K a of 2.34 and 9.60 as the p K a of its protonated amino group, we can calculate the equilibrium constant for the proton transfer reaction: 1 H 2 NCH 2 COOH H + + H 2 NCH 2 COO 2 H + + H 2 NCH 2 COO + H 3 NCH 2 COO sum : H 2 NCH 2 COOH + H 3 NCH 2 COO The equilibrium constant for such a proton transfer reaction is calculated as follows (with the subscript 1 denoting the carboxyl group and 2 denoting the amino group): p K eq = p K a1 p K a2 = 2.34 9.60 = 7.26 K eq = 10 p = 10 K eq +7.26 = 1.8 10 7 This equilibrium greatly favors the zwitterion

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isolated or fragmented CD11b signals lacking nuclear staining were excluded