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In contrast, long-term organoids are suited for high-throughput drug screening and the development of organoid databases (74, 75)

Nitric oxide assay After homogenization in a phosphate buffer, containing phenyl methyl sulfonyl fluoride as a protease inhibitor, the sample (of sciatic nerve and spinal cord) was centrifuged at 4,000g for 10 min at 4C, and the supernatant was collected and processed for determination of nitric oxide (NO) ( Histological examination For histopathological examination and extraction of the sciatic nerve, the rats were sacrificed the sixth week after DM induction

Although both glutamine and glutamate were altered in Tbx1 +/ mice, concentrations of the inhibitory neurotransmitter GABA only increased after vB12 treatment (Fig S3)

Pharmacol Res 62:282287