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Brookens, S

Membranes were incubated with primary antibodies overnight at 4 C, followed by HRP-conjugated GAPDH Rabbit mAb (#AC054, 1:5000, ABclonal) for 1 h at 25 C

Role of NLRP3 inflammasome/caspase 1 signaling pathway in cyclophosphamide-induced ovarian injury Inflammasomes, which are multiprotein complexes, assemble in the cytoplasm and are triggered by many endogenous and exogenous stimuli, including ROS and DAMPs

BDNF-Adjacent Pathways: While GHK-Cu does not directly upregulate BDNF (Brain-Derived Neurotrophic Factor), its anti-inflammatory effects in the CNS specifically its reduction of microglial activation may create conditions conducive to BDNF signaling