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Folate is critical for the development of the neural tube during the first six months of pregnancy and may help prevent neural tube defects such as spina bifida and anencephaly Folic acid helps make healthy red blood cells, which carry oxygen around the body

Statements Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Thrombin was removed by Benzamidine-Sepharose 6B resin (Cytiva) and tag-free TbTR was further purified by reverse immobilized metal affinity chromatography (Protino Ni-TED resin, Macherey-Nagel)

Triamterene-induced hyperkalemia can cause atrial fibrillation, supraventricular tachycardia (SVT) and life-threatening cardiac arrhythmias such as ventricular fibrillation, AV block, and ventricular tachycardia