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The medium was exchanged with 1.5% GelTrex in mTeSR1 (Day-2), mTeSR1 (Day-1), RPMI (Thermo Fisher Scientific) + 12 M CHIR99021 (Tocris Bioscience, Bristol, UK) (Day 0), or RPMI + B27 supplement (Thermo Fisher Scientific) (Day-1.5), and the cells were fed every 23 days to promote kidney organoid differentiation

cytokine array was used, showing that several molecules involved in T helper 1 (Th1) (pro-inflammatory) and/or Th2 (more protective) polarization, were increased in PBMC from AMN patients ( Fig