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Description

After imaging baseline morphology for 1530 min at 6 min intervals, neurons were treated with 10 mL of glycine-stimulating solution (143 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 0 mM MgCl 2 , 30 mM glucose, 10 mM HEPES, pH 7.4, 0.5 M TTX, 1 M strychnine, 20 M bicuculline and 200 M glycine) for 35 min, followed by 10 mL of 0 mM MgCl 2 containing ACSF (143 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 0 mM MgCl 2 , 30 mM glucose, 10 mM HEPES, pH 7.4, 0.5 M TTX, 1 M strychnine, and 20 M bicuculline)