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However, when the protein misfolding burden exceeds the capacity of HSPs, autophagy is activated as a secondary defense mechanism

To analyse the mitochondrial inner membrane potential in human iPSC-derived cardiomyocytes (Axol 2505), the cells were incubated in HEPES buffer (113 mM NaCl, 4.7 mM KCl, 12 mM HEPES, 1.2 mM MgSO 4 , 30 mM taurine, and 1.3 mM CaCl 2 , pH 7.4) under 1% O 2 at 37 C for 2 h