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KEGG: kyoto encyclopedia of genes and genomes

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SYBR-based quantitative PCR was conducted with the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific) with specific primers for murine IL6 or HPRT (Thermo Fisher Scientific)

Aging cells cannot be stimulating proliferation, it is by a variety of types of damage and stress caused by, not due to terminal differentiation, its characteristic is that through the cell cycle dependent kinase inhibitor p16 and p21CIP1, results in the stagnation of irreversible cell cycle ( Senescent cells can perturb their microenvironment by directly secreting senescence-related factors or by utilizing other means of communication, such as extracellular vesicles, leading to functional degradation (61)