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Research protocols typically use dosing ranges of 200-500 mcg with flexible dosing schedules to optimize for research goals

Int J Endocrinol, 2014

The cut-off point ranges from 3 to 5.1 g/L when hypoglycemia is 30 kg/m2) and over-weight (BMI 25 to 30 kg/m2) patients with a low pretest probability, we recommend a lower cutoff of 1 g/L to avoid false-positive diagnosis, because obesity physiologically reduces GH responses to stimulation tests (44)

off-target candidates/off-targets, PAM restrictiveness, target cleavage efficiency, or effector protein specificity is identified or determined using a sequencing-based double-strand break (DSB) detection assay, such as described herein elsewhere