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Additionally, our in vitro model lacks the complex multicellular and immune environment as well as mechanical forces in the lung, which play a crucial role in lung fibrogenesis and fibrosis progression

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Nucleus pulposus cells, while theoretically most advantageous due to their ability to adapt to the harsh intradiscal environment and synthesize specific matrix, are limited in number in degenerated discs, their acquisition is invasive, and they are difficult to use for repairing the annulus fibrosus and endplate, limiting clinical application