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Medical Record Documentation Providers must document both: The medically necessary E/M service The procedure performed Documentation must support the medical necessity of using Modifier-25 Documentation need not be submitted with the initial claim but should be maintained for potential review Claims Processing Guidelines Current Claims When submitting claims with Modifier-25: Documentation must justify both services E/M service must be separately identifiable Modifier-25 must be appended to the E/M code Prior Claims Carriers will not retroactively recover payments for previously processed claims

J Vis Exp 4 , 57522 (2018)

After quantification of protein concentration using a BCA kit (#G2026, Servicebio, Wuhan, China), proteins (10 g) were separated by SDS-PAGE and transferred onto PVDF membranes

It is intended for handling by trained personnel following standard laboratory protocols