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Description

Therefore, although GSH is potentially able to reduce non-native disulphide bonds in newly synthesized proteins, there is a kinetic advantage for this reduction to be catalysed by the ER oxidoreductases

However , some local side effects are possible

The GSH released from mixed disulfides by reduction with dithiothreitol is then labeled with the fluorescent probe monobromobimane and quantified by HPLC