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Secondary biological in vitro assay RT-PCR Human hair follicle dermal papilla cells (Promocell, Heidelberg, Germany, used at passages 25) were seeded into 60 mm dishes at a density of 3 10 4 cells/well with DMEM high glucose serum medium supplemented with 10% FBS and 1% antibiotic-antimycotic solution

Secondly, this study is an observational study, and therefore, the causality could not be concluded

Cancer Research 58: 4497504

The disorder is differentiated from Wilsons disease by the absence of Kayser-Fleischer rings, a variable age at onset and normal laboratory findings of serum ceruloplasmin and 24-hour urinary copper levels