The safest reconstitution method involves: (1) Using sterile technique (clean workspace, alcohol swabs, sterile syringes), (2) Using bacteriostatic water for injectionnever tap water, distilled water, or saline, (3) Allowing peptide vials to equilibrate to room temperature before reconstituting, (4) Injecting bacteriostatic water slowly down the vial wall rather than directly onto the powder, (5) Avoiding shaking or vigorous agitation, and (6) Storing reconstituted peptides refrigerated (2-8C) and protected from light
If none of your protein, or very little, is in the flow-through during your purification, then youve used sufficient glutathione agarose (Figure 5, left)
The binders with the most supportive data showing effectiveness for mycotoxins are Cholestyramine (CSM or brand Questran) 1/16 tsp every other day, increasing up to 1 scoop 4x/day Colesevelam (CSV or brand Welchol) 625mg 1/4 tab daily, increasing up to 2 tabs 3x/day taken with food has 1/4 the binding capacity as CSM Other binders Activated charcoal portion of 500mg capsule daily, increasing up to 1 cap 3x/day Bentonite clay 1/16 tsp liquid, increasing up to 1 cap 3x/day Chlorella 200mg, 1/8 tablet every other day, increasing up to 1 cap 3x/day Optifiber lean 1/4 scoop daily, increasing up to 1 scoop 3x/day Saccharomyces boulardi 1/4 capsule with food daily, increasing up 1 capsule 3x/day with meals Mycotoxins bind best to certain binders Ochratoxins cholestyramine, colesevelam, charcoal Trichothecenes / Aflotoxins charcoal, bentonite clay, chlorella Gliotoxins bentonite clay, saccharomyces boulardii, N-acetyl-cysteine Zearalanone / Enniatin B: bentonite clay, saccharomyces boulardii Dietary and environmental considerations Key is to avoid giving molds the sugars they need to survive
(2022) creates a specific problem for dosing frequency
