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1d), likely because of its intrinsic lability and some extent of spontaneous unfolding over the 60-min period of the experiment

Single-cell cloning was performed directly after sorting for GFP + cells in 96-well plates, which were then selected based on the target protein expression and/or sequencing

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Emerging therapeutic strategies, ranging from immune modulation and intestinal barrier restoration to microbiota intervention, metabolic pathway targeting, and ROS scavenging, have been developed to address core pathogenic mechanisms of IBD, thereby establishing a multi-dimensional, precision-oriented therapeutic landscape