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After overnight cultivation, the cells were inoculated in the second preculture media in 15 ml of MSM supplemented with 50 mM glucose, 0.2% casein amino acids, 15 g/ml gentamicin, and 10 M AHL to induce galA expression, as well as 1 mM vanillate to induce native vanAB expression, with initial OD 0.10.2

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