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The defensin-related pseudogenes show premature stop codons, have start-codon mutations or miss different conserved amino acids

However, the invasive method and high mortality rate are two drawbacks of this model

The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)