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In fact, due to its microenvironment dependence, tryptophan is an excellent intrinsic probe for the analysis of conformational changes of proteins upon binding of ligands 38

For example, disease-associated microglia (DAMs) play a dual role in neurodegeneration, clearing toxic aggregates such as amyloid-beta (A) in Alzheimers disease (AD) and sustaining chronic inflammation that contributes to neuronal damage [82]

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