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employed intraperitoneal injections of sodium lactate in MI model mice, revealing that lactate induces the lactylation of Snail1, subsequently promoting endothelial-to-mesenchymal transition following myocardial infarction [160]

However, in HEK293T cells, proteins with Kbhb sites are more abundant in the nucleus, followed by the mitochondria, while in MEF cells most of the Kbhb proteins were located in the cytosol (Huang et al., 2021

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Remember that some buffers change pH upon dilution or freeze-thaw cycles