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Comhaire, F

Panda B, Chilvery S, Devi P, Kalmegh R, Godugu C

The significant increase in pro-inflammatory cytokines in control cows may be attributed to a higher degree of calving stress and inflammatory condition as compared to the micronutrient injected cows which are in accordance with previous reports showing higher levels of pro-inflammatory cytokines in cows suffering from postpartum reproductive problems (5)

ROS and glutathione measurement For ROS measurement, T cells were incubated at 37 C for 20 min in PBS containing DCFHDA (1:1000, #S0033S, Beyotime, China) for cellular ROS detection or with Mito-SOX (1:200, #M36008, Invitrogen, USA) for mitochondrial ROS detection