glutathione sepharose 4b gst-tagged protein purification resin Glutathione-Tag Products 8 and 22ml respectively Glutathione Sepharose 4B GST-tagged protein

For histone H2b, Epitalon bound at site 3 near the N-terminal alpha helix, formed 12 docking solutions and binding via the sequence Val-Glu-Thr-Ser-Asn-Ser-Asn with an energy of 23.10 kcal/mol

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One is the fluid inside cells (intracellular, about 70% of the total fluids) and the other is the fluid outside cells (extracellular, about 30% of total fluids)

The recombinant vectors were then transfected into cells, which were cultured in medium containing 4 mM or 0 mM Gln, respectively, followed by detection of luciferase activity