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The next day, cells were strained through a 35-m nylon mesh and sorted on a BD FACSAria Fusion (BD Biosciences) operated on BD FACSDiva software (version 8.0.2) using a 70-m nozzle excluding aggregate, dead (Zombie NIR-positive), Cas9-negative (GFP-negative) and sgRNA library-negative (Thy1.1APC-negative) cells

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Drugs@FDA: Egrifta approval history and supplements (NDA 022505)

In addition, IGF-I induces vasodilatation[279, 321, 322], thereby influencing the regulation of vascular tone and arterial blood pressure and preserving coronary flow reserve[279, 321], platelet function, and glucose uptake[323]