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Conclusions: FeSe 2 nanozymes exhibit great promise as a biocatalyst for enhancing the shelf life of fruits and vegetables by reducing damage due to early melanogenesis

Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

The levels of IL-6, TNF-, and IFN- in the tumor homogenates were measured using commercially available ELISA kits (BioLegend, USA) according to the manufacturers instructions